By P. D. Bridge, D. K. Arora, C. A. Reddy, R. P. Elander

Polymerase chain response (PCR), a robust approach for amplifying DNA fragments, has had a major effect on examine all through biology. This e-book discusses the wide-ranging functions of PCR in natural and utilized mycology.

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1988), because it lowers the melting temperature of the dsDNA and hence facilitates strand separation. , 1990). However, the use of DMSO is not generally recommended, as it apparently increases the solubility of the mineral oil (frequently used as a protectant against evaporation) in the aqueous phase and thereby inhibits DNA polymerase (Linz, 1991). An alternative to the mineral oil layer is the use of a thermocycler with an integrated heated lid. , 1990). Glycerol is also frequently included in the reaction system (5–20%, w/v), because it may improve the yield of the amplicon by stabilizing the DNA polymerase (Instruction Manual for Pfu DNA Polymerase; Stratagene, La Jolla, California).

M. (1989) Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proceedings of the National Academy of Sciences USA 86, 232–236. Silver, J. (1991) Inverse polymerase chain reaction. , Quirke, P. R. (eds) PCR: a Practical Approach. IRL Press, Oxford, pp. 137–146. , Bainbridge, B. and Cohen, J. (1993) Detection of Aspergillus fumigatus by polymerase chain reaction. Journal of Clinical Microbiology 31, 615–621.

Journal of Clinical Microbiology 31, 2274–2280. N. and Robb, J. (1994) Use of polymerase chain reaction-amplified ribosomal intergenic sequences for the diagnosis of Verticillium tricorpus. Phytopathology 84, 256–259. E. G. (1996) Amplified fragment length polymorphism (AFLP) fingerprinting of symbiotic fungi cultured by the fungus-growing ant Cyphomyrmex minutus. Molecular Ecology 5, 119–122. B. A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology 155, 335–350.

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