By P. D. Bridge, D. K. Arora, C. A. Reddy, R. P. Elander
Polymerase chain response (PCR), a robust approach for amplifying DNA fragments, has had a major effect on examine all through biology. This e-book discusses the wide-ranging functions of PCR in natural and utilized mycology.
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Extra info for Applications of PCR in Mycology
1988), because it lowers the melting temperature of the dsDNA and hence facilitates strand separation. , 1990). However, the use of DMSO is not generally recommended, as it apparently increases the solubility of the mineral oil (frequently used as a protectant against evaporation) in the aqueous phase and thereby inhibits DNA polymerase (Linz, 1991). An alternative to the mineral oil layer is the use of a thermocycler with an integrated heated lid. , 1990). Glycerol is also frequently included in the reaction system (5–20%, w/v), because it may improve the yield of the amplicon by stabilizing the DNA polymerase (Instruction Manual for Pfu DNA Polymerase; Stratagene, La Jolla, California).
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Journal of Clinical Microbiology 31, 2274–2280. N. and Robb, J. (1994) Use of polymerase chain reaction-amplified ribosomal intergenic sequences for the diagnosis of Verticillium tricorpus. Phytopathology 84, 256–259. E. G. (1996) Amplified fragment length polymorphism (AFLP) fingerprinting of symbiotic fungi cultured by the fungus-growing ant Cyphomyrmex minutus. Molecular Ecology 5, 119–122. B. A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology 155, 335–350.